My Account: Log In | Join | Renew
Search
Author
Title
Vol.
Issue
Year
1st Page

Abstract

 

This article in CS

  1. Vol. 46 No. 5, p. 2022-2032
     
    Received: Mar 18, 2006
    Published: Sept, 2006


    * Corresponding author(s): sk_sen55@yahoo.co.in
 View
 Download
 Alerts
 Permissions

doi:10.2135/cropsci2005.11.0418

Transgenic Expression of Onion Leaf Lectin Gene in Indian Mustard Offers Protection against Aphid Colonization

  1. Munshi Azad Hossain,
  2. Mrinal K. Maiti,
  3. Asitava Basu,
  4. Supriya Sen,
  5. Arnab K. Ghosh and
  6. Soumitra K. Sen *
  1. IIT-BREF Biotek, Indian Institute of Technology, Kharagpur 721302, India

Abstract

Genes of three naturally occurring monocot mannose-binding lectins from snowdrop (Galanthus nivalis L. agglutinin, GNA), garlic (Allium sativum L. leaf agglutinin, ASAL), and onion (Allium cepa L. agglutinin, ACA) and a recombinant fusion lectin between ASAL and ACA genes were expressed in a bacterial system. The pure and active form of the recombinant lectin peptides were utilized for estimation of their sensitivity potential against feeding nymphs of mustard aphid [Lipaphis erysimi (Kaltenbach)], a major sap-sucking insect pest of Indian mustard [Brassica juncea (L.) Czern.], an oilseed crop. The artificial diet bioassay revealed that ACA and the fusion lectin contained higher toxicity potential than GNA and ASAL. Ectopic expression of these lectins in mustard plants confirmed their protective capacity on the development of the population of aphids on transgenic plants. Based on the strong possibilities that lectins originating from diverse sources would have differential insecticidal potential against different insects, deployment of the appropriate lectin gene figures as crucial in the transgenic approach to protect crop plants against sap-sucking insect pests.

  Please view the pdf by using the Full Text (PDF) link under 'View' to the left.

Copyright © 2006. Crop Science Society of AmericaCrop Science Society of America

Facebook   Twitter